Meri’s Super Duper Awesome Project

Because counting cells one by one is not Meri’s idea of a good time.

Meri at the microscope — “Simple and accessible.” — Victor Ambros

start here ↓

Experiment setup

Batch name

Name it something future-you will understand at 2 a.m.

Tissue

Cell type of interest

Tph1⁺ / 5-HT⁺ — the gut’s serotonin factories

Markers

GFP488 nm

reporter, e.g. Gch1-GFP

Count

Red561 nm

5-HTP, Atf3-tdTomato, …

Count

DAPI405 nm

nuclei counterstain

Stain

DAPI is the counterstain here — it finds nuclei, not suspects. GFP⁺ and Red⁺ cells in the same spot are scored as double-positive and show up yellow, just like in your merge channel.

Images

0 / 4

Drop your cells here.

One TIFF per channel (DAPI, GFP, red, merge), up to 4 image sets. Whole folders work too. We promise not to judge your microscopy.

TIFF · one file per channel · scale read from the file

Add images to get going.